Coding

Part:BBa_K1371003:Design

Designed by: Yiran Wu   Group: iGEM14_SCUT-China   (2014-10-03)

DH-ER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 507
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 352
    Illegal NgoMIV site found at 907
    Illegal NgoMIV site found at 1431
    Illegal NgoMIV site found at 1528
    Illegal NgoMIV site found at 1615
    Illegal NgoMIV site found at 1720
    Illegal NgoMIV site found at 1936
    Illegal AgeI site found at 1081
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Saccharopolyspora erythraea

References

[1]David E. Cane, Programming of Erythromycin Biosynthesis by a Modular Polyketide Synthase, J. Biol. Chem. (2010) 285:27517-27523.

[2]Blaine A. Pfeifer, Suzanne J. Admiraal, Hugo Gramajo, David E. Cane, Chaitan Khosla. Biosythesis of Complex Polyketides in a Metabolically Engineered Strain of, E.coli [J]. Science, 2001, 291(44): 1790-1792.

[3]Tae, Hongseok,Development of an Analysis Program of Type I Polyketide Synthase Gene Clusters Using Homology Search and Profile Hidden Markov Model, J. Microbiol. Biotechnol. (2009), 19(2), 140–146.

[4]Patrick Caffrey, Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes Chemistry & Biology 8 (2001) 713-723.